|
| Status |
Public on Dec 14, 2009 |
| Title |
LuRKO d19 testis + 2.5mg testosterone - 24hB |
| Sample type |
RNA |
| |
|
| Source name |
Testis, LH receptor knockout, 19 days post partum, treated with 2.5mg testosterone, response timecourse
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: LuRKO
tissue: testis
age post partum: 19 days + 24 hrs
testosterone dose: 2.5mg
time post testosterone injection: 24 hrs
|
| Treatment protocol |
For the two rescue series, testosterone propionate was administered as a single intraperitoneal injection at 19 days post partum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with TRI reagent, followed by clean-up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
no additional information
|
| Data processing |
The data were normalised using quantile normalisation with BeadStudio
|
| |
|
| Submission date |
Dec 14, 2009 |
| Last update date |
Dec 14, 2009 |
| Contact name |
Peter James Ellis |
| E-mail(s) |
p.j.i.ellis@kent.ac.uk
|
| Phone |
07808167834
|
| Organization name |
University of Kent
|
| Department |
School of Biosciences
|
| Street address |
Stacey Building
|
| City |
Canterbury |
| State/province |
Kent |
| ZIP/Postal code |
CT2 7NJ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE19453 |
Transcriptional profiling of luteinising hormone receptor deficient mice before and after testosterone treatment |
|
