|
| Status |
Public on May 01, 2011 |
| Title |
Heart AJ uninfected 1 |
| Sample type |
RNA |
| |
|
| Source name |
Heart mock infection
|
| Organism |
Mus musculus |
| Characteristics |
Sex: Female
strain: A/J
age: 7 weeks
tissue: heart
|
| Treatment protocol |
Mice were infected with a dose of 400pfu per gram of CVB3(CG) or mock infected with sterile PBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Hearts were perfused with st. thomas solution, removed and placed immediately into RNAlater (Ambion) RNA stabilization reagent. 30 mg were used to extract total RNA using Qiagen RNeasy Fibrous tissue, quality control by agilent bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina TotalPrep RNA Amplification Kit (IL1791).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
SVL002-1
|
| Data processing |
Raw data was imported into FlexArray (http://genomequebec.mcgill.ca/FlexArray/) and processed with a log2, RMA and loess normalization, implemented within FlexArray through the 'lumi' bioconductor package.
|
| |
|
| Submission date |
Dec 15, 2009 |
| Last update date |
May 01, 2011 |
| Contact name |
Sean A Wiltshire |
| Organization name |
McGill University
|
| Department |
Human Genetics
|
| Lab |
Silvia Vidal
|
| Street address |
3649 Sir william Osler, Rm 356
|
| City |
Montreal |
| ZIP/Postal code |
H3G 0B1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE19496 |
Coxsackievirus B3 (CVB3) infection of AJ, B10.A, CSS3 and B6.chr3AJ (heart tissue day 4) |
|
