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Sample GSM486258 Query DataSets for GSM486258
Status Public on May 01, 2011
Title Heart B10.A uninfected 2
Sample type RNA
 
Source name Heart mock infection
Organism Mus musculus
Characteristics Sex: Female
strain: B10.A(H2a)
age: 7 weeks
tissue: heart
Treatment protocol Mice were infected with a dose of 400pfu per gram of CVB3(CG) or mock infected with sterile PBS.
Extracted molecule total RNA
Extraction protocol Hearts were perfused with st. thomas solution, removed and placed immediately into RNAlater (Ambion) RNA stabilization reagent. 30 mg were used to extract total RNA using Qiagen RNeasy Fibrous tissue, quality control by agilent bioanalyzer.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion Illumina TotalPrep RNA Amplification Kit (IL1791).
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 2
SVL013-1
Data processing Raw data was imported into FlexArray (http://genomequebec.mcgill.ca/FlexArray/) and processed with a log2, RMA and loess normalization, implemented within FlexArray through the 'lumi' bioconductor package.
 
Submission date Dec 15, 2009
Last update date May 01, 2011
Contact name Sean A Wiltshire
Organization name McGill University
Department Human Genetics
Lab Silvia Vidal
Street address 3649 Sir william Osler, Rm 356
City Montreal
ZIP/Postal code H3G 0B1
Country Canada
 
Platform ID GPL6887
Series (1)
GSE19496 Coxsackievirus B3 (CVB3) infection of AJ, B10.A, CSS3 and B6.chr3AJ (heart tissue day 4)

Data table header descriptions
ID_REF
VALUE log2 RMA loess normalized signal intensity

Data table
ID_REF VALUE
ILMN_1250052 7.575394
ILMN_1251402 3.32836
ILMN_3122480 3.916586
ILMN_1230863 2.50846
ILMN_2599935 9.202899
ILMN_1249762 4.675092
ILMN_2653194 5.345926
ILMN_1227991 2.613371
ILMN_2445236 4.49025
ILMN_2675543 3.738774
ILMN_2635314 3.038239
ILMN_1212991 8.510571
ILMN_2686883 3.695056
ILMN_1223534 3.508704
ILMN_2514305 3.489734
ILMN_2751818 3.980164
ILMN_1217583 4.533563
ILMN_1222597 3.162054
ILMN_1242561 2.903885
ILMN_2728634 4.728025

Total number of rows: 45281

Table truncated, full table size 967 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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