|
| Status |
Public on Jan 30, 2010 |
| Title |
P90/P30 expression_b |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
P90 animal
|
| Organism |
Mus musculus |
| Characteristics |
age: 90 postnatal days
tissue: colonic mucosa
strain: C57BL/6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
tissue_isolation_protocol: Colonic mucosa was sheared by microscope slide and flash frozen on dry ice
Total RNA was extracted by Qiagen Rneasy mini kit (Cat.no.74106)
|
| Label |
Cy3
|
| Label protocol |
total RNA (0.4ug) samples were processed and labelled with Cy3 (Quick Amp labelling kit, two color, Agilent technologies)
|
| |
|
| Channel 2 |
| Source name |
P30 animal
|
| Organism |
Mus musculus |
| Characteristics |
age: 30 postnatal days
tissue: colonic mucosa
strain: C57BL/6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
tissue_isolation_protocol: Colonic mucosa was sheared by microscope slide and flash frozen on dry ice
Total RNA was extracted by Qiagen Rneasy mini kit (Cat.no.74106)
|
| Label |
Cy5
|
| Label protocol |
total RNA (0.4ug) samples were processed and labelled with Cy5 (Quick Amp labelling kit, two color, Agilent technologies)
|
| |
|
| |
| Hybridization protocol |
Hybridization of the arrays was carried out according to the Agilent Chip-on-chipmammalian protocol (version 9.1, 2006)
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner.
Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).
|
| Description |
none
|
| Data processing |
Agilent Feature Extraction Software (v 8.5.1.1) was used for background subtraction and LOWESS normalization. Probe ratios were calculated for P90/P30 comparisons in each of the 4 arrays separately.
|
| |
|
| Submission date |
Dec 15, 2009 |
| Last update date |
Dec 16, 2009 |
| Contact name |
Richard Kellermayer |
| Organization name |
Baylor College of Medicine
|
| Street address |
One Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE19506 |
Comparison of murine colonic mucosal gene expression between postanatal day 90 (P90) to postnatal day 30 (P30) |
|
