|
| Status |
Public on Dec 18, 2009 |
| Title |
Spleen_uninfected_control_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Spleen homogenate, uninfected
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: Female
age: 6 weeks
tissue: Spleen
infection/agent: none
|
| Treatment protocol |
Mice were euthanized and spleen tissue was collected and stored in RNAlater for microarray analysis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from spleen tissue by phase separation with Trizol (Invitrogen, USA) and column chromatography using an Rneasy Mini kit (Qiagen Inc, USA), RNA quantity was measured using a Nanodrop (Nanodrop Technologies, USA) and quality was assessed using an Agilent Bioanalyzer Nanochip (Agilent Technologies, USA)
|
| Label |
Cy3
|
| Label protocol |
cRNA was synthesised from 250ng of pooled total RNA with an Illumina Total Prep RNA Amplification Kit (Ambion Inc., USA) according to the manufacturers instructions
|
| |
|
| Hybridization protocol |
cRNA was hybridised to Illumina mouse-6 v1.1 expression beadchips according to the manufacturers instructions
|
| Scan protocol |
Microarrays were scanned using a Beadstation 500 at Green factor level 2
|
| Description |
Gene expression in the spleen of normal C57BL/6 mice
|
| Data processing |
Data was analysed using BeadStudio and Genespring GX; Values reported were derived using Illumina BeadStudio v3 and represent relative signal intensity normalized to background, Genespring GX was used to normalise each chip to the 50th percentile and each gene to the median and uninfected controls, measurements less than 0.01 were set to 0.01
|
| |
|
| Submission date |
Dec 17, 2009 |
| Last update date |
Dec 17, 2009 |
| Contact name |
Melissa Louise Burke |
| E-mail(s) |
melissa.burke@qimr.edu.au
|
| Organization name |
Queensland Institute of Medical Research
|
| Department |
Division of Infectious Diseases and Immunology
|
| Lab |
Molecular Parasitology
|
| Street address |
300 Herston Road
|
| City |
Herston |
| State/province |
QLD |
| ZIP/Postal code |
4006 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6481 |
| Series (1) |
| GSE19525 |
Gene expression in the Schistosoma japonicum infected spleen |
|
