|
| Status |
Public on Jan 01, 2010 |
| Title |
IL-17-non-producing hybridoma DE3-2 |
| Sample type |
RNA |
| |
|
| Source name |
IL-17-non-producing hybridoma
|
| Organism |
Mus musculus |
| Characteristics |
cell type: hybridoma
|
| Growth protocol |
hybridomas were maintained in RPMI supplimented with 10% fetal calf serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Rneasy Mini Kit(QIAGEN). Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
n/a
|
| Data processing |
The data were normalized using robust spline normalization in R
|
| |
|
| Submission date |
Dec 17, 2009 |
| Last update date |
Dec 17, 2009 |
| Contact name |
Kensuke Shibata |
| E-mail(s) |
k_shibata@bioreg.kyushu-u.ac.jp
|
| Phone |
81-92-642-6962
|
| Fax |
81-92-642-6973
|
| Organization name |
Kyushu University
|
| Department |
Medical Institute of Bioregulation
|
| Lab |
host defense
|
| Street address |
3-1-1, Maidashi, Higashi-ku
|
| City |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE19527 |
Microarray analysis of transcriptional regulations in IL-17-producing hybridoma |
|
