|
| Status |
Public on Dec 23, 2009 |
| Title |
Qinchuan cattle, longuissimus dorsi muscle at 36 months, biological rep CM3 |
| Sample type |
RNA |
| |
|
| Source name |
Qinchuan cattle, longuissimus dorsi muscle, castrated male, 36 months
|
| Organism |
Bos taurus |
| Characteristics |
breed: Qinchuan
tissue: longuissimus dorsi muscle
gender: castrated male
age: 36 months
|
| Treatment protocol |
Cattle were slaughtered at 36 months of age. Longuissimus dorsi tissues were taken adjacent to the 12th section of the Longuissimus dorsi muscle, immediately frozen in liquid nitrogen, and conserved at -80C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Bovine Genome arrays using ArrayAffymetrix® GeneChip Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix® GeneChip® Scanner 3000.
|
| Description |
Gene expression data from Qinchuan cattle longuissimus dorsi muscle at 36 months.
|
| Data processing |
The data were analyzed with Affymetrix® GeneChip® Operating Software Version1.4 (GCOS1.4) using Affymetrix default analysis settings. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Dec 19, 2009 |
| Last update date |
Dec 22, 2009 |
| Contact name |
Yingying Zhang |
| E-mail(s) |
zyysingbird@yahoo.com.cn
|
| Organization name |
National Beef Cattle Improvement Center
|
| Street address |
No.22 Xinong Road, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.
|
| City |
Yangling |
| ZIP/Postal code |
712100 |
| Country |
China |
| |
|
| Platform ID |
GPL2112 |
| Series (1) |
| GSE19586 |
Expression data from the longuissimus dorsi muscle of Qinchuan cattle, Qinghai yak and Guangxi buffalo at 36 months |
|
