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Sample GSM488133 Query DataSets for GSM488133
Status Public on Apr 08, 2011
Title Luciferase replicate 1
Sample type RNA
 
Source name Luciferase replicate 1
Organism Mus musculus
Characteristics cell type: Embryonic Stem Cell
transfection: LucR +dox
Growth protocol rtTA-expressing ES cells Aniv15 were used for derivation of rescue clones and were maintained on primary mouse embryonic fibroblasts with DMEM media supplemented with 15% FBS, 100 mM MEM non-essential amino acids, 0.1 mM 2-mercaptoethanol, 1 mM l-glutamine and 103 U ml-1 of LIF (Chemicon) and doxycycline (1ug/ml)
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label Cy3
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Luciferase replicate 1
Data processing Data was VST normalized in R using LUMI and LIMMA packages
 
Submission date Dec 20, 2009
Last update date Apr 08, 2011
Contact name Jonathan Monk
E-mail(s) jonathan.m.monk@gmail.com
Phone 9716457785
Organization name Mt. Sinai School of Medicine
Department Gene and Cell Medicine
Lab Lemischka
Street address One Gustave L. Levy Place
City New York
State/province NY
ZIP/Postal code 10029
Country USA
 
Platform ID GPL6885
Series (1)
GSE19588 Wdr5 mediates self-renewal and reprogramming via the embryonic stem cell core transcriptional network (2)

Data table header descriptions
ID_REF
VALUE VST normalized

Data table
ID_REF VALUE
ILMN_2659724 7.703349143
ILMN_2706226 7.684400449
ILMN_1217123 7.639100321
ILMN_2780205 13.51659989
ILMN_2713160 7.632372166
ILMN_2650094 7.663186367
ILMN_2703479 7.649908067
ILMN_2952493 7.688408784
ILMN_2731592 7.91400774
ILMN_2729743 7.958417067
ILMN_1252621 8.543885067
ILMN_3162407 9.694816589
ILMN_2692952 7.794325001
ILMN_2910076 7.67130191
ILMN_2709047 9.109757262
ILMN_2718462 8.055929968
ILMN_2882078 10.24549541
ILMN_2882079 8.538956788
ILMN_2771368 7.615686106
ILMN_2724039 7.709779208

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Raw data are available on Series record
Processed data are available on Series record

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