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Sample GSM488135 Query DataSets for GSM488135
Status Public on Apr 08, 2011
Title Rescue Clone Minus Dox replicate 1
Sample type RNA
 
Source name Rescue Clone Minus Dox replicate 1
Organism Mus musculus
Characteristics cell type: Embryonic Stem Cell
transfection: Wdr5R4 -dox
Growth protocol rtTA-expressing ES cells Aniv15 were used for derivation of rescue clones and were maintained on primary mouse embryonic fibroblasts with DMEM media supplemented with 15% FBS, 100 mM MEM non-essential amino acids, 0.1 mM 2-mercaptoethanol, 1 mM l-glutamine and 103 U ml-1 of LIF (Chemicon) and doxycycline (1ug/ml)
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label Cy3
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Wdr5 KD through shRNA expression
Data processing Data was VST normalized in R using LUMI and LIMMA packages
 
Submission date Dec 20, 2009
Last update date Apr 08, 2011
Contact name Jonathan Monk
E-mail(s) jonathan.m.monk@gmail.com
Phone 9716457785
Organization name Mt. Sinai School of Medicine
Department Gene and Cell Medicine
Lab Lemischka
Street address One Gustave L. Levy Place
City New York
State/province NY
ZIP/Postal code 10029
Country USA
 
Platform ID GPL6885
Series (1)
GSE19588 Wdr5 mediates self-renewal and reprogramming via the embryonic stem cell core transcriptional network (2)

Data table header descriptions
ID_REF
VALUE VST normalized

Data table
ID_REF VALUE
ILMN_2659724 7.664451138
ILMN_2706226 7.635040837
ILMN_1217123 7.659486006
ILMN_2780205 14.16697975
ILMN_2713160 7.652363763
ILMN_2650094 7.655096501
ILMN_2703479 7.65400245
ILMN_2952493 7.640966329
ILMN_2731592 8.016067943
ILMN_2729743 8.281340522
ILMN_1252621 9.164993384
ILMN_3162407 10.40877964
ILMN_2692952 7.80476614
ILMN_2910076 7.704209112
ILMN_2709047 9.17018226
ILMN_2718462 8.276757122
ILMN_2882078 9.9155808
ILMN_2882079 8.509177514
ILMN_2771368 7.640966329
ILMN_2724039 7.673915233

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Raw data are available on Series record
Processed data are available on Series record

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