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Sample GSM488136 Query DataSets for GSM488136
Status Public on Apr 08, 2011
Title Rescue Clone Minus Dox replicate 2
Sample type RNA
 
Source name Rescue Clone Minus Dox replicate 2
Organism Mus musculus
Characteristics cell type: Embryonic Stem Cell
transfection: Wdr5R4 -dox
Growth protocol rtTA-expressing ES cells Aniv15 were used for derivation of rescue clones and were maintained on primary mouse embryonic fibroblasts with DMEM media supplemented with 15% FBS, 100 mM MEM non-essential amino acids, 0.1 mM 2-mercaptoethanol, 1 mM l-glutamine and 103 U ml-1 of LIF (Chemicon) and doxycycline (1ug/ml)
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label Cy3
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Wdr5 KD through shRNA expression
Data processing Data was VST normalized in R using LUMI and LIMMA packages
 
Submission date Dec 20, 2009
Last update date Apr 08, 2011
Contact name Jonathan Monk
E-mail(s) jonathan.m.monk@gmail.com
Phone 9716457785
Organization name Mt. Sinai School of Medicine
Department Gene and Cell Medicine
Lab Lemischka
Street address One Gustave L. Levy Place
City New York
State/province NY
ZIP/Postal code 10029
Country USA
 
Platform ID GPL6885
Series (1)
GSE19588 Wdr5 mediates self-renewal and reprogramming via the embryonic stem cell core transcriptional network (2)

Data table header descriptions
ID_REF
VALUE VST normalized

Data table
ID_REF VALUE
ILMN_2659724 7.64015173
ILMN_2706226 7.647777658
ILMN_1217123 7.649160598
ILMN_2780205 14.07590557
ILMN_2713160 7.6577183
ILMN_2650094 7.637107389
ILMN_2703479 7.651962487
ILMN_2952493 7.693682878
ILMN_2731592 8.085024756
ILMN_2729743 8.141980102
ILMN_1252621 9.165136747
ILMN_3162407 10.36314219
ILMN_2692952 7.917099851
ILMN_2910076 7.661675329
ILMN_2709047 9.107759263
ILMN_2718462 8.111183944
ILMN_2882078 10.01148373
ILMN_2882079 8.575668566
ILMN_2771368 7.6577183
ILMN_2724039 7.737828523

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Raw data are available on Series record
Processed data are available on Series record

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