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Sample GSM489045 Query DataSets for GSM489045
Status Public on Dec 23, 2009
Title WT-OVA-8-D1-2
Sample type RNA
 
Source name WT
Organism Mus musculus
Characteristics tissue: whole lung
genome/variation: WT
treatment group: OVA treated
treatment time: 1 d after last treatment
Treatment protocol WT or CC-LR mice were either challenged with NTHi lysate or sensitized to ovalbumin by intraperitoneal injection weekly for two weeks, then challenged for 30 min to an aerosol of ovalbumin in 0.9% saline weekly for eight weeks. Mice from each group were euthanized on day one after the last exposure for comparison. To reduce the lung leukocyte burden, the pulmonary vasculature was perfused and the airways lavaged with PBS.
Extracted molecule total RNA
Extraction protocol The lungs were mechanically homogenized, then total RNA was isolated from lung homogenates using the RNeasy system (Qiagen, Valencia, CA), and cRNA was synthesized and amplified from equal masses of total RNA using the Ilumina TotalPrep RNA amplification kit (Ambion, Austin, TX).
Label Cy3
Label protocol cRNA was hybridized to BeadChips prior to labeling with Cy3-Streptavidin per Illumina instructions.
 
Hybridization protocol cRNA was hybridized to BeadChips per Illumina instructions.
Scan protocol Chips were scanned using level 3 signal using a BeadStation 500 scanner.
Description WT, OVA treated
Data processing Data was processed using Illumina BeadStudio v3. Matrix tables were generated both without normalization and with rank invariant normalization, using sham group as the reference group. Values reported were derived using Illumina BeadStudio v3 and are relative signal intensity normalized to background.
 
Submission date Dec 22, 2009
Last update date Dec 22, 2009
Contact name Scott E Evans
E-mail(s) seevans@mdanderson.org
Organization name University of Texas-M.D. Anderson Cancer Center
Department Pulmonary Medicine - Box 403
Street address 1400 Holcombe Blvd
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6885
Series (1)
GSE19605 Specificity for the Nature of Inflammation in Lung Cancer Promotion in Mice

Data table header descriptions
ID_REF
VALUE rank invariant normalized (BeadStudio v3) signal intensity

Data table
ID_REF VALUE
ILMN_2896528 940.7971
ILMN_2721178 244.0545
ILMN_3033922 90.32748
ILMN_3092673 2211.426
ILMN_2816356 -56.27082
ILMN_2808939 196.7221
ILMN_2634564 869.4064
ILMN_2737647 69.01821
ILMN_2734484 109.2713
ILMN_2952292 143.2546
ILMN_2699078 0.8317303
ILMN_1213681 178.4608
ILMN_2735413 -70.65454
ILMN_2735415 -71.00945
ILMN_2891688 281.0014
ILMN_2637698 612.3769
ILMN_2674228 104.805
ILMN_2601546 -18.12091
ILMN_1230831 -103.9425
ILMN_2848071 16.39906

Total number of rows: 25697

Table truncated, full table size 559 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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