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Sample GSM489262 Query DataSets for GSM489262
Status Public on Jun 01, 2011
Title Cystic Fibrosis Replicate One
Sample type RNA
 
Channel 1
Source name BALB CF knockout
Organism Mus musculus
Characteristics genotype: Balbc/J Cftrtm1UNC -/-
gender: male
tissue type: Ileum
Growth protocol All mice were maintained on Colyte from 3 weeks to age of euthanasia, at 12 weeks
Extracted molecule total RNA
Extraction protocol Total RNA from mouse ileum was extracted using miRNeasy Mini kits from Qiagen according to manufacturer's protocol.
Label Hy3
Label protocol 1 μg of total RNA from sample and reference was labelled with Hy3TM and Hy5TM respectively using miRCURYTM LNA Array power labelling kit from Exiqon according to the manufacturer's protocol.
 
Channel 2
Source name Control RNA from Exiqon
Organism Mus musculus
Characteristics reference: Control RNA from Exiqon
Growth protocol All mice were maintained on Colyte from 3 weeks to age of euthanasia, at 12 weeks
Extracted molecule total RNA
Extraction protocol Total RNA from mouse ileum was extracted using miRNeasy Mini kits from Qiagen according to manufacturer's protocol.
Label Hy5
Label protocol 1 μg of total RNA from sample and reference was labelled with Hy3TM and Hy5TM respectively using miRCURYTM LNA Array power labelling kit from Exiqon according to the manufacturer's protocol.
 
 
Hybridization protocol The Hy3TM and Hy5TM labelled samples were mixed pairwise and hybridization was performed according to the miRCURYTM LNA array manual (Exiqon) using a Tecan HS4800 hybridization station
Scan protocol Microarray slides were scanned using the Agilent G2565BA Microarray Scanner System. Image Analysis used ImaGene 8.0 software.
Description raw data file Hy3: 1_Exiqon_13978181_S01_Cropped.txt
raw data file Hy5: 0_Exiqon_13978181_S01_Cropped.txt
Biological Replicate 1 of 8 for BALB CF mice
Data processing LOWESS normalized and background corrected (Normexp with offset value 10)
 
Submission date Dec 23, 2009
Last update date Jun 01, 2011
Contact name Christina Kathleen Haston
E-mail(s) christina.haston@mcgill.ca
Phone 514-398-3864 089714
Organization name McGill University
Department Medicine
Street address 3626 St. Urbain
City Montreal
State/province QC
ZIP/Postal code H2X 2P2
Country Canada
 
Platform ID GPL7723
Series (1)
GSE19621 MicroRNA profiling of cystic fibrosis intestinal disease in mice

Data table header descriptions
ID_REF
VALUE log2(Hy3/Hy5) ratios, representing sample/reference

Data table
ID_REF VALUE
14306 0.412787108492
11078 0.682711309581
10986 0.143100371701
19582 0.507403375537
11208 0.831665404236
19599 0.697811471688
46621 0.427627804359
46793 0.645840139187
42724 0.473177399847
10998 0.739372297813
46730 0.745174833847
42471 0.292305621247
10967 0.692548211582
5740 0.356677014836
13143 0.579452937725
10985 0.829217104924
27575 0.787127467048
46636 0.521177254675
10997 0.680308147084
42682 0.667998504893

Total number of rows: 657

Table truncated, full table size 10 Kbytes.




Supplementary file Size Download File type/resource
GSM489262_0_Exiqon_13978181_S01_Cropped.txt.gz 787.3 Kb (ftp)(http) TXT
GSM489262_1_Exiqon_13978181_S01_Cropped.txt.gz 869.9 Kb (ftp)(http) TXT
Processed data included within Sample table

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