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Sample GSM489266 Query DataSets for GSM489266
Status Public on Jun 01, 2011
Title Cystic Fibrosis Replicate Five
Sample type RNA
 
Channel 1
Source name BALB CF knockout
Organism Mus musculus
Characteristics genotype: Balbc/J Cftrtm1UNC -/-
gender: female
tissue type: Ileum
Growth protocol All mice were maintained on Colyte from 3 weeks to age of euthanasia, at 12 weeks
Extracted molecule total RNA
Extraction protocol Total RNA from mouse ileum was extracted using miRNeasy Mini kits from Qiagen according to manufacturer's protocol.
Label Hy3
Label protocol 1 μg of total RNA from sample and reference was labelled with Hy3TM and Hy5TM respectively using miRCURYTM LNA Array power labelling kit from Exiqon according to the manufacturer's protocol.
 
Channel 2
Source name Control RNA from Exiqon
Organism Mus musculus
Characteristics reference: Control RNA from Exiqon
Growth protocol All mice were maintained on Colyte from 3 weeks to age of euthanasia, at 12 weeks
Extracted molecule total RNA
Extraction protocol Total RNA from mouse ileum was extracted using miRNeasy Mini kits from Qiagen according to manufacturer's protocol.
Label Hy5
Label protocol 1 μg of total RNA from sample and reference was labelled with Hy3TM and Hy5TM respectively using miRCURYTM LNA Array power labelling kit from Exiqon according to the manufacturer's protocol.
 
 
Hybridization protocol The Hy3TM and Hy5TM labelled samples were mixed pairwise and hybridization was performed according to the miRCURYTM LNA array manual (Exiqon) using a Tecan HS4800 hybridization station
Scan protocol Microarray slides were scanned using the Agilent G2565BA Microarray Scanner System. Image Analysis used ImaGene 8.0 software.
Description raw data file Hy3: 1_Exiqon_13978186_S01_Cropped.txt
raw data file Hy5: 0_Exiqon_13978186_S01_Cropped.txt
Biological Replicate 5 of 8 for BALB CF mice
Data processing LOWESS normalized and background corrected (Normexp with offset value 10)
 
Submission date Dec 23, 2009
Last update date Jun 01, 2011
Contact name Christina Kathleen Haston
E-mail(s) christina.haston@mcgill.ca
Phone 514-398-3864 089714
Organization name McGill University
Department Medicine
Street address 3626 St. Urbain
City Montreal
State/province QC
ZIP/Postal code H2X 2P2
Country Canada
 
Platform ID GPL7723
Series (1)
GSE19621 MicroRNA profiling of cystic fibrosis intestinal disease in mice

Data table header descriptions
ID_REF
VALUE log2(Hy3/Hy5) ratios, representing sample/reference

Data table
ID_REF VALUE
14306 0.542068932044
11078 0.952878911131
10986 0.748412345926
19582 0.616101933960
11208 0.878066669189
19599 0.671562674439
46621 0.695036282856
46793 0.584970691427
42724 0.488219273113
10998 0.553726353752
46730 0.687983525453
42471 0.651132181518
10967 0.697420487079
5740 0.691038188489
13143 0.412926671679
10985 0.697764631676
27575 0.578744134684
46636 0.600703082401
10997 0.486978694732
42682 0.752137296080

Total number of rows: 657

Table truncated, full table size 10 Kbytes.




Supplementary file Size Download File type/resource
GSM489266_0_Exiqon_13978186_S01_Cropped.txt.gz 799.2 Kb (ftp)(http) TXT
GSM489266_1_Exiqon_13978186_S01_Cropped.txt.gz 876.5 Kb (ftp)(http) TXT
Processed data included within Sample table

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