|
| Status |
Public on Jun 01, 2011 |
| Title |
Wild Type Replicate Two |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
BALB WT Control
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Balbc/J Cftrtm1UNC +/+
gender: male
tissue type: Ileum
|
| Growth protocol |
All mice were maintained on Colyte from 3 weeks to age of euthanasia, at 12 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from mouse ileum was extracted using miRNeasy Mini kits from Qiagen according to manufacturer's protocol.
|
| Label |
Hy3
|
| Label protocol |
1 μg of total RNA from sample and reference was labelled with Hy3TM and Hy5TM respectively using miRCURYTM LNA Array power labelling kit from Exiqon according to the manufacturer's protocol.
|
| |
|
| Channel 2 |
| Source name |
Control RNA from Exiqon
|
| Organism |
Mus musculus |
| Characteristics |
reference: Control RNA from Exiqon
|
| Growth protocol |
All mice were maintained on Colyte from 3 weeks to age of euthanasia, at 12 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from mouse ileum was extracted using miRNeasy Mini kits from Qiagen according to manufacturer's protocol.
|
| Label |
Hy5
|
| Label protocol |
1 μg of total RNA from sample and reference was labelled with Hy3TM and Hy5TM respectively using miRCURYTM LNA Array power labelling kit from Exiqon according to the manufacturer's protocol.
|
| |
|
| |
| Hybridization protocol |
The Hy3TM and Hy5TM labelled samples were mixed pairwise and hybridization was performed according to the miRCURYTM LNA array manual (Exiqon) using a Tecan HS4800 hybridization station
|
| Scan protocol |
Microarray slides were scanned using the Agilent G2565BA Microarray Scanner System. Image Analysis used ImaGene 8.0 software.
|
| Description |
raw data file Hy3: 1_Exiqon_13978194_S01_Cropped.txt
raw data file Hy5: 0_Exiqon_13978194_S01_Cropped.txt
Biological Replicate 2 of 7 for BALB WT mice
|
| Data processing |
LOWESS normalized and background corrected (Normexp with offset value 10)
|
| |
|
| Submission date |
Dec 23, 2009 |
| Last update date |
Jun 01, 2011 |
| Contact name |
Christina Kathleen Haston |
| E-mail(s) |
christina.haston@mcgill.ca
|
| Phone |
514-398-3864 089714
|
| Organization name |
McGill University
|
| Department |
Medicine
|
| Street address |
3626 St. Urbain
|
| City |
Montreal |
| State/province |
QC |
| ZIP/Postal code |
H2X 2P2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL7723 |
| Series (1) |
| GSE19621 |
MicroRNA profiling of cystic fibrosis intestinal disease in mice |
|
