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Sample GSM489315 Query DataSets for GSM489315
Status Public on Sep 01, 2010
Title mesenteric arterial tree, eET-1 mouse, replicate 3, female mature
Sample type RNA
Source name mesenteric arterial tree, eET-1 mouse
Organism Mus musculus
Characteristics strain: C57BL/6
genome/variation: eET-1
gender: female
age: 6.7 months
tissue: mesenteric arterial tree
Extracted molecule total RNA
Extraction protocol Tissue was collected and stored in RNAlater overnight at 4°C with gentle agitation. Fat was removed from tissue in RNA later. RNA was extracted from cleaned arteries with Trizol reagent and chlorofom, followed by Qiagen RNeasy MinElute Cleanup Kit spin columns in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyzer.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Illumina Total Prep RNA amplification kit for Illumina arrays
Hybridization protocol standard Illumina hybridization protocol
Scan protocol standard Illumina scanning protocol
Description FEMALE MATURE 3
Data processing The data was normalised using the lumi algorithm in FlexArray analysis software (RMA background adjustment, variance stabililizing tranformation, robust splin normalization)
Submission date Dec 23, 2009
Last update date Feb 27, 2010
Contact name Stefania Simeone
Phone 514-340-8222
Organization name Lady Davis Institute for Medical Research
Department Hypertension and Vascular Research Unit
Lab Dr. Ernesto L Schiffrin
Street address 3755 Côte Ste-Catherine Road
City Montreal
State/province Quebec
ZIP/Postal code H3T 1E2
Country Canada
Platform ID GPL6885
Series (1)
GSE19624 Vascular gene expression in mice overexpressing human endothelin-1 targeted to the endothelium

Data table header descriptions
VALUE RMA, VST, robust splin normalized signal intensity

Data table
ILMN_1250052 8.139418
ILMN_3122480 7.998375
ILMN_2599935 8.706815
ILMN_2675543 8.002263
ILMN_2686883 8.042498
ILMN_2751818 8.008894
ILMN_2728634 8.108953
ILMN_3040515 8.033685
ILMN_2711608 7.989151
ILMN_1232875 8.245071
ILMN_1258507 8.003047
ILMN_2746142 7.9958
ILMN_1252690 8.051258
ILMN_2655499 7.988631
ILMN_1252870 8.019298
ILMN_1248179 7.99097
ILMN_2649955 8.318072
ILMN_2628708 8.11436
ILMN_3024781 8.004331
ILMN_2705628 8.042125

Total number of rows: 25697

Table truncated, full table size 549 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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