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Sample GSM489317 Query DataSets for GSM489317
Status Public on Sep 01, 2010
Title mesenteric arterial tree, WT mouse, replicate 1, female mature
Sample type RNA
 
Source name mesenteric arterial tree, WT mouse
Organism Mus musculus
Characteristics strain: C57BL/6
genome/variation: wild type
gender: female
age: 6.6 months
tissue: mesenteric arterial tree
Extracted molecule total RNA
Extraction protocol Tissue was collected and stored in RNAlater overnight at 4°C with gentle agitation. Fat was removed from tissue in RNA later. RNA was extracted from cleaned arteries with Trizol reagent and chlorofom, followed by Qiagen RNeasy MinElute Cleanup Kit spin columns in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyzer.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Illumina Total Prep RNA amplification kit for Illumina arrays
 
Hybridization protocol standard Illumina hybridization protocol
Scan protocol standard Illumina scanning protocol
Description FEMALE MATURE 5
Data processing The data was normalised using the lumi algorithm in FlexArray analysis software (RMA background adjustment, variance stabililizing tranformation, robust splin normalization)
 
Submission date Dec 23, 2009
Last update date Feb 27, 2010
Contact name Stefania Simeone
E-mail(s) stefania.simeone@mail.mcgill.ca
Phone 514-340-8222
Organization name Lady Davis Institute for Medical Research
Department Hypertension and Vascular Research Unit
Lab Dr. Ernesto L Schiffrin
Street address 3755 Côte Ste-Catherine Road
City Montreal
State/province Quebec
ZIP/Postal code H3T 1E2
Country Canada
 
Platform ID GPL6885
Series (1)
GSE19624 Vascular gene expression in mice overexpressing human endothelin-1 targeted to the endothelium

Data table header descriptions
ID_REF
VALUE RMA, VST, robust splin normalized signal intensity

Data table
ID_REF VALUE
ILMN_1250052 8.074847
ILMN_3122480 7.995792
ILMN_2599935 8.99235
ILMN_2675543 7.992358
ILMN_2686883 8.060191
ILMN_2751818 7.996912
ILMN_2728634 8.077086
ILMN_3040515 8.017329
ILMN_2711608 7.997279
ILMN_1232875 8.188399
ILMN_1258507 7.994418
ILMN_2746142 7.987077
ILMN_1252690 8.099911
ILMN_2655499 7.997435
ILMN_1252870 8.06275
ILMN_1248179 8.018274
ILMN_2649955 8.249706
ILMN_2628708 8.0736
ILMN_3024781 7.990273
ILMN_2705628 8.149035

Total number of rows: 25697

Table truncated, full table size 549 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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