|
| Status |
Public on Sep 01, 2010 |
| Title |
mesenteric arterial tree, eET-1 mouse, replicate 3, male mature |
| Sample type |
RNA |
| |
|
| Source name |
mesenteric arterial tree, eET-1 mouse
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genome/variation: eET-1
gender: male
age: 6.7 months
tissue: mesenteric arterial tree
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tissue was collected and stored in RNAlater overnight at 4°C with gentle agitation. Fat was removed from tissue in RNA later. RNA was extracted from cleaned arteries with Trizol reagent and chlorofom, followed by Qiagen RNeasy MinElute Cleanup Kit spin columns in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina Total Prep RNA amplification kit for Illumina arrays
|
| |
|
| Hybridization protocol |
standard Illumina hybridization protocol
|
| Scan protocol |
standard Illumina scanning protocol
|
| Description |
MALE MATURE 3
|
| Data processing |
The data was normalised using the lumi algorithm in FlexArray analysis software (RMA background adjustment, variance stabililizing tranformation, robust splin normalization)
|
| |
|
| Submission date |
Dec 23, 2009 |
| Last update date |
Feb 27, 2010 |
| Contact name |
Stefania Simeone |
| E-mail(s) |
stefania.simeone@mail.mcgill.ca
|
| Phone |
514-340-8222
|
| Organization name |
Lady Davis Institute for Medical Research
|
| Department |
Hypertension and Vascular Research Unit
|
| Lab |
Dr. Ernesto L Schiffrin
|
| Street address |
3755 Côte Ste-Catherine Road
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3T 1E2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE19624 |
Vascular gene expression in mice overexpressing human endothelin-1 targeted to the endothelium |
|
