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Sample GSM489333 Query DataSets for GSM489333
Status Public on Sep 01, 2010
Title mesenteric arterial tree, WT mouse, replicate 1, male mature
Sample type RNA
 
Source name mesenteric arterial tree, WT mouse
Organism Mus musculus
Characteristics strain: C57BL/6
genome/variation: wild type
gender: male
age: 7 months
tissue: mesenteric arterial tree
Extracted molecule total RNA
Extraction protocol Tissue was collected and stored in RNAlater overnight at 4°C with gentle agitation. Fat was removed from tissue in RNA later. RNA was extracted from cleaned arteries with Trizol reagent and chlorofom, followed by Qiagen RNeasy MinElute Cleanup Kit spin columns in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyzer.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Illumina Total Prep RNA amplification kit for Illumina arrays
 
Hybridization protocol standard Illumina hybridization protocol
Scan protocol standard Illumina scanning protocol
Description MALE MATURE 5
Data processing The data was normalised using the lumi algorithm in FlexArray analysis software (RMA background adjustment, variance stabililizing tranformation, robust splin normalization)
 
Submission date Dec 23, 2009
Last update date Feb 27, 2010
Contact name Stefania Simeone
E-mail(s) stefania.simeone@mail.mcgill.ca
Phone 514-340-8222
Organization name Lady Davis Institute for Medical Research
Department Hypertension and Vascular Research Unit
Lab Dr. Ernesto L Schiffrin
Street address 3755 Côte Ste-Catherine Road
City Montreal
State/province Quebec
ZIP/Postal code H3T 1E2
Country Canada
 
Platform ID GPL6885
Series (1)
GSE19624 Vascular gene expression in mice overexpressing human endothelin-1 targeted to the endothelium

Data table header descriptions
ID_REF
VALUE RMA, VST, robust splin normalized signal intensity

Data table
ID_REF VALUE
ILMN_1250052 7.965453
ILMN_3122480 7.847959
ILMN_2599935 8.47242
ILMN_2675543 7.846524
ILMN_2686883 8.036072
ILMN_2751818 7.841782
ILMN_2728634 7.9972
ILMN_3040515 7.853356
ILMN_2711608 7.844446
ILMN_1232875 7.953835
ILMN_1258507 7.856961
ILMN_2746142 7.832028
ILMN_1252690 7.862912
ILMN_2655499 7.842363
ILMN_1252870 7.846013
ILMN_1248179 7.877686
ILMN_2649955 8.153646
ILMN_2628708 7.876846
ILMN_3024781 7.847633
ILMN_2705628 7.922826

Total number of rows: 25697

Table truncated, full table size 549 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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