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Sample GSM490186 Query DataSets for GSM490186
Status Public on Dec 24, 2009
Title Pituitary Rb1+/--3
Sample type RNA
Source name Pituitary tumor
Organism Mus musculus
Characteristics tissue: pituitary gland
disease state: pituitary tumor
genotype/variation: Rb1+/-
strain: (CD-1 x C57BL/6) F1 hybrid background
age: 12 months
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Replicate 3
Data processing Z score transformation for normalization was performed for each Illumina sample/array as previously described (Cheadle et al. J Mol Diagn, 5:73-81).
Submission date Dec 24, 2009
Last update date Dec 24, 2009
Contact name Madson Q Almeida
Phone 301 451 3768
Fax 301 402 0574
Organization name NIH
Department Section on Genetics and Endocrinology / NICHD
Lab Dr. Stratakis Lab
Street address 10 Center Drive, MSC 1103
City Bethesda
State/province MD
ZIP/Postal code 20892-1103
Country USA
Platform ID GPL6885
Series (1)
GSE19576 Prkar1a haploinsufficiency leads to an overall increase in tumors caused by other genetic defects or chemical induction

Data table header descriptions
VALUE Z-score
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 1.497153033 0
ILMN_2721178 -0.009101502 0
ILMN_1227723 -0.078242135 0
ILMN_3033922 0.782003649 0
ILMN_3092673 5.543583881 0
ILMN_2730714 3.230395498 0
ILMN_3162224 0.24184932 0
ILMN_2808939 -0.000527623 0
ILMN_2634564 -0.087255699 0
ILMN_1216623 0.136654429 0
ILMN_1215157 0.400575989 0
ILMN_2734484 0.775408358 0
ILMN_2952292 -0.151559786 0
ILMN_2660815 0.383538154 0
ILMN_1243593 0.660760217 0
ILMN_1219797 -0.175412755 0
ILMN_1231132 -0.109679689 0
ILMN_2718462 -0.167608328 0
ILMN_2653881 -0.002176446 0
ILMN_2638061 -0.181128674 0

Total number of rows: 25697

Table truncated, full table size 687 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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