|
| Status |
Public on Dec 24, 2009 |
| Title |
Sarcoma Prkar1a+/- Tp53+/--2 |
| Sample type |
RNA |
| |
|
| Source name |
Sarcoma
|
| Organism |
Mus musculus |
| Characteristics |
tissue: toe bone
disease state: toe osteosarcoma
genotype/variation: Prkar1a+/- Tp53+/-
strain: (CD-1 x C57BL/6) F1 hybrid background
age: 12 months
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Replicate 2
|
| Data processing |
Z score transformation for normalization was performed for each Illumina sample/array as previously described (Cheadle et al. J Mol Diagn, 5:73-81).
|
| |
|
| Submission date |
Dec 24, 2009 |
| Last update date |
Dec 24, 2009 |
| Contact name |
Madson Q Almeida |
| E-mail(s) |
madsonalmeida@usp.br
|
| Phone |
301 451 3768
|
| Fax |
301 402 0574
|
| Organization name |
NIH
|
| Department |
Section on Genetics and Endocrinology / NICHD
|
| Lab |
Dr. Stratakis Lab
|
| Street address |
10 Center Drive, MSC 1103
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892-1103 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE19576 |
Prkar1a haploinsufficiency leads to an overall increase in tumors caused by other genetic defects or chemical induction |
|
