|
| Status |
Public on Oct 18, 2010 |
| Title |
Ref1.2 (Agilent) |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA with small fraction
|
| Organism |
Mus musculus |
| Characteristics |
sample type: pool of equal amounts of total RNA from mouse testicle, ovary and 10-12 day embryo.
microarray barcode: 251911910767
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA containing the small fraction was isolated from each sample using the miRVana miRNA Isolation Kit (Ambion), Streetsville, ON, Canada). Quality was confirmed using the Agilent 2100 Bioanalyzer and RNA Nano 6000 chips.
|
| Label |
Cy3
|
| Label protocol |
Total RNA samples were labelled using the Agilent miRNA Complete Labelling and Hybridisation Kit (Agilent Tech, Mississauga, ON, Canada).
|
| |
|
| Hybridization protocol |
Hybridisations were performed in Agilent SureHyb chambers for 20 hours at 55C and washed according to manufacturer's instructions.
|
| Scan protocol |
Arrays were scanned at a resolution of 5 mm using an Agilent G2505B scanner.
|
| Description |
Reference RNA was developed from FirstChoice® mouse Total RNA including the small fraction (Catalogue # AM7800-AM7828, Ambion, Streetsville, ON)
|
| Data processing |
Data were extracted from scanned images using Agilent Feature Extraction software version 9.5.3.1. Median signal intensities were cyclic lowess normalized. Technical replicates for each miRNA level were averaged using the median. Data was flagged if the raw signal intensity was less than the mean plus three standard deviations of the negative controls or if the median of the technical replicates was flagged to be in the background.
|
| |
|
| Submission date |
Dec 28, 2009 |
| Last update date |
Oct 18, 2010 |
| Contact name |
Andrew Williams |
| Organization name |
Health Canada
|
| Street address |
50 Columbine
|
| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1A OK9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL8824 |
| Series (1) |
| GSE19669 |
Cross-platform correlation analysis of global microRNA expression technologies |
|
