|
| Status |
Public on May 24, 2021 |
| Title |
Muscle_Control_1 |
| Sample type |
RNA |
| |
|
| Source name |
Gastrocnemius muscle of mice fed a Control diet
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
strain: C57BL/6J
tissue: Gastrocnemius
food: D12450B
age: 21 months
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Fibrous Tissue Mini Kit (Qiagen, Hilden, Germany) was used to extract skeletal muscle total RNA. Total RNA was extracted from the tissue cryopreserved with liquid nitrogen according to the kit’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was synthesized by GeneChip 3' IVT PLUS Reagent Kit Thermo Fisher Scientific) from 100 ng total RNA according to the manufacturer's instructions. Biotinylated cRNA yield was checked with the NanoDrop ND-2000 Spectrophotometer.
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA was hybridized for 16 hr at 45˚C on GeneChip Mouse Genome 430 2.0 Array. GeneChip array was washed and stained in the GeneChip Fluidics Station 450 (Thermo Fisher Scientific).
|
| Scan protocol |
GeneChip array was scanned using GeneChip Scanner 3000 7G.
|
| Data processing |
Single Array Analysis was calculated by Microarray Suite version 5.0 (MAS5.0) with Thermo Fisher Scientific default setting and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Nov 17, 2020 |
| Last update date |
May 24, 2021 |
| Contact name |
Naho Kitamura |
| E-mail(s) |
nahoshi@sfc.keio.ac.jp
|
| Organization name |
Keio University
|
| Department |
Graduate School of Media and Governance
|
| Street address |
5322, Endo
|
| City |
Fujisawa |
| State/province |
Kanagawa |
| ZIP/Postal code |
252-0882 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE161646 |
Iron supplementation regulates the progression of high fat diet induced obesity and hepatic steatosis via mitochondrial signaling pathways [II] |
|
