|
| Status |
Public on Apr 01, 2010 |
| Title |
WT, mouse 1, 0 LPS |
| Sample type |
RNA |
| |
|
| Source name |
peritoneal macrophage
|
| Organism |
Mus musculus |
| Characteristics |
time point: 0 LPS
genotype: Wt
cell type: peritoneal macrophage
|
| Treatment protocol |
100 ng/ml LPS (E. coli serotype O55:B5, Sigma) for 6 hours, or untreated
|
| Growth protocol |
Peritoneal macrophages prepared from three threereplicates of three wild type and three Tyk2 deficient mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRiZOL
|
| Label |
Cy5
|
| Label protocol |
CodeLink protocol: from ds cDNA in vitro transcription and biotin labelling of cRNA
|
| |
|
| Hybridization protocol |
CodeLink protocol: Streptavidin-Cy5 staining
|
| Scan protocol |
GenePix Array scanner 4000B; GenePix Pro 4.0 (Axon Instruments, Inc.)
|
| Description |
n/a
|
| Data processing |
CodeLink Expression Analysis software (GE Healthcare); values below 25 and unannotated genes filtered out; log transformed values adjusted to mean of zero and standard deviation of one within each sample
|
| |
|
| Submission date |
Jan 04, 2010 |
| Last update date |
Jan 04, 2010 |
| Contact name |
Claus Vogl |
| E-mail(s) |
claus.vogl@vetmeduni.ac.at
|
| Phone |
+43-1-250775631
|
| Organization name |
University of Veterinary Medicine, Vienna
|
| Department |
Biomedical Sciences
|
| Lab |
Animal Breeding and Genetics
|
| Street address |
Veterinaerplatz 1
|
| City |
Vienna |
| State/province |
Vienna |
| ZIP/Postal code |
A-1210 |
| Country |
Austria |
| |
|
| Platform ID |
GPL8063 |
| Series (1) |
| GSE19733 |
Transcriptome analysis reveals a major impact of Tyk2 on the expression of interferon responsive and metabolic genes |
|
