|
Status |
Public on Apr 01, 2010 |
Title |
Tyk2-/-, mouse 2, 0 LPS |
Sample type |
RNA |
|
|
Source name |
peritoneal macrophage
|
Organism |
Mus musculus |
Characteristics |
time point: 0 LPS genotype: Tyk2-/- cell type: peritoneal macrophage
|
Treatment protocol |
100 ng/ml LPS (E. coli serotype O55:B5, Sigma) for 6 hours, or untreated
|
Growth protocol |
Peritoneal macrophages prepared from three threereplicates of three wild type and three Tyk2 deficient mice
|
Extracted molecule |
total RNA |
Extraction protocol |
TRiZOL
|
Label |
Cy5
|
Label protocol |
CodeLink protocol: from ds cDNA in vitro transcription and biotin labelling of cRNA
|
|
|
Hybridization protocol |
CodeLink protocol: Streptavidin-Cy5 staining
|
Scan protocol |
GenePix Array scanner 4000B; GenePix Pro 4.0 (Axon Instruments, Inc.)
|
Description |
n/a
|
Data processing |
CodeLink Expression Analysis software (GE Healthcare); values below 25 and unannotated genes filtered out; log transformed values adjusted to mean of zero and standard deviation of one within each sample
|
|
|
Submission date |
Jan 04, 2010 |
Last update date |
Jan 04, 2010 |
Contact name |
Claus Vogl |
E-mail(s) |
claus.vogl@vetmeduni.ac.at
|
Phone |
+43-1-250775631
|
Organization name |
University of Veterinary Medicine, Vienna
|
Department |
Biomedical Sciences
|
Lab |
Animal Breeding and Genetics
|
Street address |
Veterinaerplatz 1
|
City |
Vienna |
State/province |
Vienna |
ZIP/Postal code |
A-1210 |
Country |
Austria |
|
|
Platform ID |
GPL8063 |
Series (1) |
GSE19733 |
Transcriptome analysis reveals a major impact of Tyk2 on the expression of interferon responsive and metabolic genes |
|