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Sample GSM492793 Query DataSets for GSM492793
Status Public on Apr 01, 2010
Title Tyk2-/-, mouse 2, 0 LPS
Sample type RNA
 
Source name peritoneal macrophage
Organism Mus musculus
Characteristics time point: 0 LPS
genotype: Tyk2-/-
cell type: peritoneal macrophage
Treatment protocol 100 ng/ml LPS (E. coli serotype O55:B5, Sigma) for 6 hours, or untreated
Growth protocol Peritoneal macrophages prepared from three threereplicates of three wild type and three Tyk2 deficient mice
Extracted molecule total RNA
Extraction protocol TRiZOL
Label Cy5
Label protocol CodeLink protocol: from ds cDNA in vitro transcription and biotin labelling of cRNA
 
Hybridization protocol CodeLink protocol: Streptavidin-Cy5 staining
Scan protocol GenePix Array scanner 4000B; GenePix Pro 4.0 (Axon Instruments, Inc.)
Description n/a
Data processing CodeLink Expression Analysis software (GE Healthcare); values below 25 and unannotated genes filtered out; log transformed values adjusted to mean of zero and standard deviation of one within each sample
 
Submission date Jan 04, 2010
Last update date Jan 04, 2010
Contact name Claus Vogl
E-mail(s) claus.vogl@vetmeduni.ac.at
Phone +43-1-250775631
Organization name University of Veterinary Medicine, Vienna
Department Biomedical Sciences
Lab Animal Breeding and Genetics
Street address Veterinaerplatz 1
City Vienna
State/province Vienna
ZIP/Postal code A-1210
Country Austria
 
Platform ID GPL8063
Series (1)
GSE19733 Transcriptome analysis reveals a major impact of Tyk2 on the expression of interferon responsive and metabolic genes

Data table header descriptions
ID_REF
VALUE Normalized log signal intensity

Data table
ID_REF VALUE
1
2 0.315518291
3
4
5
6 0.616540045
7
8 0.423288196
9 1.607363711
10 -0.511183462
11 -0.362544441
12
13 -0.389526817
14 -0.457644338
15 -0.907172862
16 -1.01737766
17 0.68452648
18 -0.808151565
19 -1.447550195
20

Total number of rows: 36227

Table truncated, full table size 346 Kbytes.




Supplementary file Size Download File type/resource
GSM492793_2_T00314528_TYK_0.txt.gz 1.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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