|
| Status |
Public on Dec 07, 2010 |
| Title |
Uncoated cellulose implant, 1 day, rat 3 |
| Sample type |
RNA |
| |
|
| Source name |
subcutaneous implantation of uncoated cellulose, 1 day, rat 3
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
age: 10 weeks
tissue: Granulation tissue
time: 1 day
|
| Growth protocol |
Subcutaneous implantation into rat
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from implants was isolated with RNeasy midi kit according to manufacturer´s instructions, followed by the Dnase I treatment (Promega) as recommended by manufacturer.
|
| Label |
biotin
|
| Label protocol |
For the Illumina gene expression analysis, 100 ng of purified total RNA was amplified and biotinylated in an overnight in vitro transcription reaction using the Illumina RNA TotalPrepTM amplification kit (Ambion, Applied Biosystems).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
no additional information
|
| Data processing |
The data were normalized using quantile normalisation with limma in R
|
| |
|
| Submission date |
Jan 05, 2010 |
| Last update date |
Dec 07, 2010 |
| Contact name |
Miretta Tommila |
| E-mail(s) |
miorli@utu.fi
|
| Organization name |
Turun yliopisto
|
| Department |
Lääketieteellisen biokemian ja genetiikan laitos
|
| Street address |
Kiinamyllynkatu 10
|
| City |
Turku |
| ZIP/Postal code |
20520 |
| Country |
Finland |
| |
|
| Platform ID |
GPL6101 |
| Series (1) |
| GSE19749 |
Gene expression screening during early granulation tissue formation (II) |
|
