|
| Status |
Public on Dec 31, 2010 |
| Title |
10 day old, blood feed, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Female adult, 10 day old, Blood feed
|
| Organism |
Anopheles gambiae |
| Characteristics |
tissue: whole body
genotype: G3
age: 10 day
|
| Treatment protocol |
Female mosquitoes were collected at Day 1, Day 4, Day 10, Day 19 and Day 28. All the females were split into two sets. One set of mosquitoes were not blood fed and the other set of mosquitoes were blood fed every five days. Blood-fed females only collected at Day 10, Day 19, and Day 28.
|
| Growth protocol |
There were 250 adult (male:female=1:1) for each cage. Mosquito colonies were maintained by blood feeding on anesthetized rabbits every five days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
3.75 μg of the resulting amplified cDNA material for each sample was first fragmented and subsequently end-labeled using the NuGEN FL-Ovation cDNA Biotin Module V2 (NuGEN Technologies, San Carlos CA)
|
| |
|
| Hybridization protocol |
2.2 μg of the resulting fragmented, biotin-tagged cDNA was placed into a hybridization cocktail (220 μL), with 200 μL actually hybridized at 45°C with rotation for 18 hours (Affymetrix GeneChip Hybridization Oven 640) to probe sets present on an Affymetrix array (GeneChip® Plasmodium/Anopheles Genome Array). The GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450 using the FS450_0004 fluidics protocol
|
| Scan protocol |
Scanning on an Affymetrix GeneChip 3000 Scanner 7G
|
| Description |
Female adult, 10 day old, Blood feed
|
| Data processing |
Results were quantified and analyzed using Expression Console ver.1.1 software (Affymetrix, Inc.) using the PLIER Algorithm default values (Quantification Scale: Linear; Quantification Type: Signal and Detection P-Value; Background: PM-GCBG; Normalization Method: Sketch-Quantile).
|
| |
|
| Submission date |
Jan 06, 2010 |
| Last update date |
Dec 31, 2010 |
| Contact name |
Mei-Hui Wang |
| E-mail(s) |
meihuiw@uci.edu
|
| Phone |
949-824-0249
|
| Fax |
949-824-0249
|
| Organization name |
UC irvine
|
| Street address |
3501B Hewitt Hall UCI
|
| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92697 |
| Country |
USA |
| |
|
| Platform ID |
GPL1321 |
| Series (1) |
| GSE19756 |
Gene Expression Profiling Data of African Malaria Vector Anopheles gambiae Aging |
|
