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Sample GSM494559 Query DataSets for GSM494559
Status Public on Jan 30, 2011
Title Lung Cancer 17T
Sample type RNA
 
Source name frozen tissue of primary tumor
Organism Homo sapiens
Characteristics tissue: lung cancer
gender: female
age: 55
stage: 2B
Extracted molecule total RNA
Extraction protocol Total RNA from the tissue specimens were isolated using TRIzol® reagent (Invitrogen, Calsbad, CA) and purified with RNeasy® mini kits (Qiagen) according to the manufacturer's instructions.
Label biotin
Label protocol The IVT reaction was carried out in the presence of T7 RNA Polymerase and a biotinylated nucleotide analog/ribonucleotide mix for complementary RNA (cRNA) amplification and biotin labeling.
 
Hybridization protocol The biotinylated cRNA targets were then cleaned up, fragmented, and hybridized to GeneChip® Human Genome U133 Plus 2.0 expression arrays (Affymetrix, Inc., Santa Clara, CA). After 16 hours of hybridization at 45 °C, the arrays were washed by Fluidics Station 450
Scan protocol Chips were scanned using the GeneChip Scanner 3000
Description Gene expression data from 17T
Data processing Background-adjusted signals were normalized by three steps: Robust Multichip Analysis (RMA), median polish method, and quantile normalization algorithm.
 
Submission date Jan 08, 2010
Last update date Jan 30, 2011
Contact name Tzu-Pin Lu
E-mail(s) tplu@ntu.edu.tw
Organization name National Taiwan University, Taiwan
Department Department of Public Health, Institute of Epidemiology and Preventive Medicine
Street address No. 1, Sec. 4, Roosevelt Road
City Taipei
ZIP/Postal code 10617
Country Taiwan
 
Platform ID GPL570
Series (2)
GSE19804 Genome-wide screening of transcriptional modulation in non-smoking female lung cancer in Taiwan
GSE33356 Genome-wide screening of genomic alterations and transcriptional modulation in non-smoking female lung cancer in Taiwan

Data table header descriptions
ID_REF
VALUE Log2 RMA signal intensity

Data table
ID_REF VALUE
1007_s_at 11.9036
1053_at 7.91211
117_at 7.13887
121_at 8.67404
1255_g_at 3.69171
1294_at 8.28728
1316_at 5.87546
1320_at 5.75049
1405_i_at 8.85718
1431_at 4.58431
1438_at 6.22988
1487_at 8.23779
1494_f_at 6.27847
1552256_a_at 7.99348
1552257_a_at 9.05793
1552258_at 5.35686
1552261_at 6.94612
1552263_at 7.21689
1552264_a_at 7.41033
1552266_at 4.04687

Total number of rows: 54675

Table truncated, full table size 998 Kbytes.




Supplementary file Size Download File type/resource
GSM494559.CEL.gz 5.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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