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Sample GSM494560 Query DataSets for GSM494560
Status Public on Jan 30, 2011
Title Lung Cancer 32T
Sample type RNA
 
Source name frozen tissue of primary tumor
Organism Homo sapiens
Characteristics tissue: lung cancer
gender: female
age: 65
stage: 3A
Extracted molecule total RNA
Extraction protocol Total RNA from the tissue specimens were isolated using TRIzol® reagent (Invitrogen, Calsbad, CA) and purified with RNeasy® mini kits (Qiagen) according to the manufacturer's instructions.
Label biotin
Label protocol The IVT reaction was carried out in the presence of T7 RNA Polymerase and a biotinylated nucleotide analog/ribonucleotide mix for complementary RNA (cRNA) amplification and biotin labeling.
 
Hybridization protocol The biotinylated cRNA targets were then cleaned up, fragmented, and hybridized to GeneChip® Human Genome U133 Plus 2.0 expression arrays (Affymetrix, Inc., Santa Clara, CA). After 16 hours of hybridization at 45 °C, the arrays were washed by Fluidics Station 450
Scan protocol Chips were scanned using the GeneChip Scanner 3000
Description Gene expression data from 32T
Data processing Background-adjusted signals were normalized by three steps: Robust Multichip Analysis (RMA), median polish method, and quantile normalization algorithm.
 
Submission date Jan 08, 2010
Last update date Jan 30, 2011
Contact name Tzu-Pin Lu
E-mail(s) tplu@ntu.edu.tw
Organization name National Taiwan University, Taiwan
Department Department of Public Health, Institute of Epidemiology and Preventive Medicine
Street address No. 1, Sec. 4, Roosevelt Road
City Taipei
ZIP/Postal code 10617
Country Taiwan
 
Platform ID GPL570
Series (2)
GSE19804 Genome-wide screening of transcriptional modulation in non-smoking female lung cancer in Taiwan
GSE33356 Genome-wide screening of genomic alterations and transcriptional modulation in non-smoking female lung cancer in Taiwan

Data table header descriptions
ID_REF
VALUE Log2 RMA signal intensity

Data table
ID_REF VALUE
1007_s_at 11.7523
1053_at 8.27448
117_at 7.72479
121_at 8.78145
1255_g_at 3.93509
1294_at 8.35532
1316_at 5.76576
1320_at 5.70967
1405_i_at 8.68914
1431_at 4.5152
1438_at 6.75017
1487_at 8.35433
1494_f_at 6.21242
1552256_a_at 8.64607
1552257_a_at 8.8308
1552258_at 5.42463
1552261_at 5.70888
1552263_at 6.95478
1552264_a_at 7.3744
1552266_at 4.22577

Total number of rows: 54675

Table truncated, full table size 998 Kbytes.




Supplementary file Size Download File type/resource
GSM494560.CEL.gz 4.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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