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Sample GSM494652 Query DataSets for GSM494652
Status Public on Jan 30, 2011
Title Lung Normal 133N
Sample type RNA
 
Source name frozen tissue of adjacent normal
Organism Homo sapiens
Characteristics tissue: paired normal adjacent
gender: female
age: 70
stage: n/a
Extracted molecule total RNA
Extraction protocol Total RNA from the tissue specimens were isolated using TRIzol® reagent (Invitrogen, Calsbad, CA) and purified with RNeasy® mini kits (Qiagen) according to the manufacturer's instructions.
Label biotin
Label protocol The IVT reaction was carried out in the presence of T7 RNA Polymerase and a biotinylated nucleotide analog/ribonucleotide mix for complementary RNA (cRNA) amplification and biotin labeling.
 
Hybridization protocol The biotinylated cRNA targets were then cleaned up, fragmented, and hybridized to GeneChip® Human Genome U133 Plus 2.0 expression arrays (Affymetrix, Inc., Santa Clara, CA). After 16 hours of hybridization at 45 °C, the arrays were washed by Fluidics Station 450
Scan protocol Chips were scanned using the GeneChip Scanner 3000
Description Gene expression data from 133N
Data processing Background-adjusted signals were normalized by three steps: Robust Multichip Analysis (RMA), median polish method, and quantile normalization algorithm.
 
Submission date Jan 08, 2010
Last update date Jan 30, 2011
Contact name Tzu-Pin Lu
E-mail(s) tplu@ntu.edu.tw
Organization name National Taiwan University, Taiwan
Department Department of Public Health, Institute of Epidemiology and Preventive Medicine
Street address No. 1, Sec. 4, Roosevelt Road
City Taipei
ZIP/Postal code 10617
Country Taiwan
 
Platform ID GPL570
Series (2)
GSE19804 Genome-wide screening of transcriptional modulation in non-smoking female lung cancer in Taiwan
GSE33356 Genome-wide screening of genomic alterations and transcriptional modulation in non-smoking female lung cancer in Taiwan

Data table header descriptions
ID_REF
VALUE Log2 RMA signal intensity

Data table
ID_REF VALUE
1007_s_at 10.9075
1053_at 7.5526
117_at 7.27485
121_at 8.14004
1255_g_at 3.8197
1294_at 8.78228
1316_at 5.67686
1320_at 8.13714
1405_i_at 11.5326
1431_at 4.58923
1438_at 7.34215
1487_at 8.22253
1494_f_at 6.43629
1552256_a_at 8.79513
1552257_a_at 8.19415
1552258_at 5.34725
1552261_at 5.42605
1552263_at 7.02966
1552264_a_at 9.14673
1552266_at 4.33547

Total number of rows: 54675

Table truncated, full table size 998 Kbytes.




Supplementary file Size Download File type/resource
GSM494652.CEL.gz 5.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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