|
| Status |
Public on Jan 30, 2011 |
| Title |
Lung Normal 136N |
| Sample type |
RNA |
| |
|
| Source name |
frozen tissue of adjacent normal
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: paired normal adjacent
gender: female
age: 65
stage: n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from the tissue specimens were isolated using TRIzol® reagent (Invitrogen, Calsbad, CA) and purified with RNeasy® mini kits (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The IVT reaction was carried out in the presence of T7 RNA Polymerase and a biotinylated nucleotide analog/ribonucleotide mix for complementary RNA (cRNA) amplification and biotin labeling.
|
| |
|
| Hybridization protocol |
The biotinylated cRNA targets were then cleaned up, fragmented, and hybridized to GeneChip® Human Genome U133 Plus 2.0 expression arrays (Affymetrix, Inc., Santa Clara, CA). After 16 hours of hybridization at 45 °C, the arrays were washed by Fluidics Station 450
|
| Scan protocol |
Chips were scanned using the GeneChip Scanner 3000
|
| Description |
Gene expression data from 136N
|
| Data processing |
Background-adjusted signals were normalized by three steps: Robust Multichip Analysis (RMA), median polish method, and quantile normalization algorithm.
|
| |
|
| Submission date |
Jan 08, 2010 |
| Last update date |
Jan 30, 2011 |
| Contact name |
Tzu-Pin Lu |
| E-mail(s) |
tplu@ntu.edu.tw
|
| Organization name |
National Taiwan University, Taiwan
|
| Department |
Department of Public Health, Institute of Epidemiology and Preventive Medicine
|
| Street address |
No. 1, Sec. 4, Roosevelt Road
|
| City |
Taipei |
| ZIP/Postal code |
10617 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE19804 |
Genome-wide screening of transcriptional modulation in non-smoking female lung cancer in Taiwan |
| GSE33356 |
Genome-wide screening of genomic alterations and transcriptional modulation in non-smoking female lung cancer in Taiwan |
|
