|
Status |
Public on May 17, 2010 |
Title |
NHE1-OX 2wk5 |
Sample type |
RNA |
|
|
Source name |
K-line transgenic sample
|
Organism |
Mus musculus |
Characteristics |
tissue: heart strain: FVB gender: male age: 14 days
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from frozen tissues of using RNeasy kit (Qiagen, Valencia, CA) according to the manufacturer's instructions.
|
Label |
Biotin
|
Label protocol |
Illumina® TotalPrep™ RNA Amplification Kit following the manufacturer's instructions. Input amount: 100ng of total RNA.
|
|
|
Hybridization protocol |
Hybridizations were carried out by using the Illumina gene expression system according to the manufacturer's instructions.
|
Scan protocol |
Illumina scanner
|
Description |
1888865064_A
|
Data processing |
Normalized average intensity of the bead type/target in the group using Cubic spline normalization with BeadStudio 2.3.47.
|
|
|
Submission date |
Jan 08, 2010 |
Last update date |
May 17, 2010 |
Contact name |
Dan Zhou |
E-mail(s) |
d2zhou@ucsd.edu
|
Phone |
858-822-6889
|
Fax |
858-534-6971
|
Organization name |
University of California, San Diego, School of Medicine
|
Department |
Pediatrics
|
Lab |
Haddad Lab
|
Street address |
CMG 103, 9500 Gilman Drive, MC0735
|
City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92093-0735 |
Country |
USA |
|
|
Platform ID |
GPL6481 |
Series (1) |
GSE19819 |
Elevated Myocardial Na+/H+ Exchanger Elicits the Expression of Genes that Lead to Cardiac Hypertrophy |
|