|
| Status |
Public on Jan 12, 2011 |
| Title |
liver-olive oil -4weeks-rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Liver, 4 weeks, Olive oil, replicate 1
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Liver
time: 4 weeks
administrated reagent: Olive oil
disease state: control
|
| Treatment protocol |
CCL4 or olive oil was administrated intraperitoneally in mouse twice a week for 4 weeks and then administrated once a week for four weeks. Mice were sacrificed on 4, 6, and 8 weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using mirVana miRNA isolation kit following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
miRNA microarrays were manufactured by Agilent Technologies, and 100 ng of total RNA was labeled and hybridized using Mouse microRNA Microarray Kit according to manufacture’s protocol (Protocol for use with Agilent microRNA microarrays Version 1.0)
|
| |
|
| Hybridization protocol |
miRNA microarrays were manufactured by Agilent Technologies, and 100 ng of total RNA was labeled and hybridized using Mouse microRNA Microarray Kit according to manufacture’s protocol (Protocol for use with Agilent microRNA microarrays Version 1.0)
|
| Scan protocol |
Scanned on an Agilent G2505B scanner
|
| Description |
miRNA expression after 4 weeks in olive oil-administrated mouse liver
|
| Data processing |
Data were analyzed using GeneSpring GX 7.3.1 software (Agilent Technologies) and normalized as follows: (i) Values below 0.01 were set to 0.01. (ii) Each measurement was divided by the 75th percentile of all measurements to compare one-color expression profiles.
|
| |
|
| Submission date |
Jan 12, 2010 |
| Last update date |
Jan 12, 2011 |
| Contact name |
Nobuyoshi Kosaka |
| E-mail(s) |
nkosaka@ncc.go.jp
|
| Phone |
+81-3-3542-2511
|
| Fax |
+81-3-5565-0727
|
| Organization name |
National Cancer Center Research Institute
|
| Department |
Section for Studies on Metastasis
|
| Street address |
5-1-1, Tsukiji, Chuo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
104-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL8824 |
| Series (1) |
| GSE19865 |
Aberrant expression of microRNAs is associated to the progression of liver fibrosis |
|
