|
| Status |
Public on Jan 13, 2010 |
| Title |
Brain frontal lobes of pup exposed to 0.2 mg/kg BW BDE-47 rep2 |
| Sample type |
RNA |
| |
|
| Source name |
brain fontal lobes
|
| Organism |
Rattus norvegicus |
| Characteristics |
sex: female
tissue: brain frontal lobe
|
| Treatment protocol |
Dams were randomly distributed among three groups (3 dams per group) and exposed by caudal injection (0.3 ml/kg body weight) from GD 15 to PND 20 every 5 days (a total of 6 injections/dam). Dams of the control group received vehicle: ethanol 95%, Cremophor EL, and sterile water (1:1:8, v/v); dams of exposed group 1 received 0.002 mg/kg body weight (BW) of BDE-47 in vehicle, and dams of exposed group 2 received 0.2 mg/kg body weight (BW) of BDE-47 in vehicle. Dams and pups were kept together until weaning on PND 21. One female pup per litter was selected randomly and sacrificed by decapitation on PND 41. Total RNA was extracted from brain frontal lobes of these pups and used for microarray analysis.
|
| Growth protocol |
We obtainedtimed pregnant Wistar rats (250–300 g; Charles River Laboratories, St. Constant, Que., Canada) on gestational day (GD) 14 and housed them individually in plastic cages with sawdust bedding, under regulated temperature (21 ± 2 ° C), relative humidity (50 ± 10%) and a 12-hour light/dark cycle. Food (Rodent chow 5075; Charles River Laboratories) and water were provided ad libitum. All animals received care in compliance with the Guide to the Care and Use of Experimental Animals from the Canadian Council of Animal Care and the protocol was approved by our institutional animal research ethics review board.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
All tissues were snap-frozen in liquid nitrogen, and stored at -80°C until RNA extraction. RNA was extracted with the RNeasy Mini Kit (Qiagen, Maryland, USA) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
individual pup
|
| Data processing |
The raw data were pre-processed and normalized with the lumi Bioconductor package
|
| |
|
| Submission date |
Jan 12, 2010 |
| Last update date |
Jan 12, 2010 |
| Contact name |
Alexander Suvorov |
| E-mail(s) |
alexander.suvorov@usherbrooke.ca
|
| Organization name |
Université de Sherbrooke
|
| Street address |
3001, 12e avenue Nord
|
| City |
Sherbrooke |
| State/province |
Quebec |
| ZIP/Postal code |
J1H 5N4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6101 |
| Series (1) |
| GSE19867 |
Whole brains and frontal lobes of rat offsprings exposed perinatally to low doses of 2,2',4,4'-tetrabromodiphenyl ether |
|
