|
| Status |
Public on Oct 21, 2010 |
| Title |
HFD 3 |
| Sample type |
RNA |
| |
|
| Source name |
islet from female offspring of fathers fed on high fat diet
|
| Organism |
Rattus norvegicus |
| Characteristics |
gender: Female
strain: Sprague-Dawley
|
| Treatment protocol |
Fathers fed on HFD vs control, mate with females on normal chow then the female offspring's islet transcriptomics were analysed through microarray analysis
|
| Growth protocol |
At 15 weeks of age, fathers were mated with normal females consuming chow, to generate the F1 offspring.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. (Invitrogen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Gene ST 1.0 GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression from islet
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100. The data is then normalised using RMA in log 2 space and differentially expressed genes were selected based on ANOVA of chow (Control) vs high fat diet (HFD).
|
| |
|
| Submission date |
Jan 13, 2010 |
| Last update date |
Oct 17, 2010 |
| Contact name |
Margaret J Morris |
| E-mail(s) |
m.morris@unsw.edu.au
|
| Phone |
+61293851560
|
| Organization name |
University of New South Wales
|
| Department |
School of Medical Sciences
|
| Lab |
Head of Pharmacology
|
| Street address |
University of New South Wales
|
| City |
Wallace Wurth Building, Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2052 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE19877 |
Effects of dietary obesity in fathers on gene expression of islets in the female offspring |
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