|
| Status |
Public on Jan 15, 2010 |
| Title |
hypoxic_P12.5_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
hypoxic_P12.5_rep3
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6/N
tissue type: retina
age: postnatal day12.5
|
| Growth protocol |
Litters of 7-day-old [postnatal day 7 (P7)] C57BL/6N pups along with their mothers were placed in a 75 ± 2% oxygen atmosphere for 5 days and then returned to room air at age P12
|
| Extracted molecule |
total RNA |
| Extraction protocol |
To extract the RNAs, retinas isolated from normal P12 mice, P12 and P12.5 hyperoxic/hypoxic exposed mice were homogenized with a Polytron homogenizer in extraction reagent, extracted with chloroform, and the aqueous phase was precipitated in isopropanol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
none
|
| Data processing |
Raw gene expression data were summarized using the Illumina Bead Studio software. Normalization (quantile method), calculation of signal intensities and fold change analysis between groups were performed by Genespring v 7.3
|
| |
|
| Submission date |
Jan 14, 2010 |
| Last update date |
Jan 14, 2010 |
| Contact name |
Keijiro Ishikawa |
| E-mail(s) |
keijishi@med.kyushu-u.ac.jp
|
| Organization name |
Kyushu University
|
| Department |
Ophthalmology
|
| Street address |
3-1-1 Maidashi, Higashi-ku
|
| City |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6481 |
| Series (1) |
| GSE19886 |
Gene Expression Profile of Hyperoxic/hypoxic Retinas in Mouse Model of Oxygen-induced Retinopathy |
|
