|
| Status |
Public on Oct 06, 2021 |
| Title |
MH-Ctrl-2 |
| Sample type |
RNA |
| |
|
| Source name |
Control
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: macrophages
treatment: control
|
| Treatment protocol |
the cell lines were treated with control and HMEOs as descripted in the manuscript.
|
| Growth protocol |
The human macrophages derived by PMA induced were cultured as descripted in the manuscipt.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The quality and quantity of the RNA were tested using Bioanalyzer (Agilent Technologies, Santa Clara, CA). 4.5 μg of RNA from each sample was used for target labeling by a two-round amplification protocol (Expression Analysis Technical Manual, 20012, Affymetrix).
|
| |
|
| Hybridization protocol |
GeneChips were scanned using the Affymetrix GeneAtlas hyberzation station according to the manufacturer's instructions.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneAtlas Fluidic station according to the manufacturer's instructions.
|
| Description |
The whole transcriptome Gene expression profile data
|
| Data processing |
The data were analyzed with Affymetrix Expression Console using Robust Mutichip Average (RMA) preprocessing followed by “per chip” normalization across all samples. All Affymetrix control genes were removed from the expression data and the remaining Affymetrix probe clusters were imported into the Affymetrix Power Tools software (APT package).
|
| |
|
| Submission date |
Dec 13, 2020 |
| Last update date |
Oct 06, 2021 |
| Contact name |
shubai Liu |
| E-mail(s) |
shubliu@gmail.com
|
| Organization name |
kunming institute of botany, chinese academic of science
|
| Street address |
132 lanhei road,
|
| City |
kunming |
| State/province |
yunnan |
| ZIP/Postal code |
650223 |
| Country |
China |
| |
|
| Platform ID |
GPL11532 |
| Series (1) |
| GSE163125 |
The whole transcriptome expression profiling of human macrophages; comparison between control and human milk exosomes capsulated oligosaccharides treatment |
|
