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Status |
Public on Aug 18, 2010 |
Title |
TAIL3_WT |
Sample type |
RNA |
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Source name |
Zebrafish Tail Mesoderm 21-somite stage
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Organism |
Danio rerio |
Characteristics |
developmental stage: 21-somite genotype: wild-type
|
Treatment protocol |
No treatments were given to the fish -- comparisons were made between wild-type and mutant individuals. Mutants harbored the b104 mutant allele of the transcription factor tbx16/spt.
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Growth protocol |
Fish were grown and cared for following standard techniques (Westerfield M: The zebrafish book: a guide for the laboratory use of zebrafish (Danio rerio): University of Oregon Press Eugene, OR; 1995)
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Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using the PicoPure isolation kit (Arcturus)
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Label |
biotin
|
Label protocol |
Biotin following standard Affymetrix protocols by the University of Chicago functional genomics core facility
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|
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Hybridization protocol |
Following standard Affymetrix protocols by the University of Chicago functional genomics core facility
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Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
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Description |
Gene expression data from 21-somite stage tail tissue
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Data processing |
Image acquisition and initial array quantification were performed using the Affymetrix Microarray Suite Version 5.0. Subsequent identification of differentially expressed genes between wild-type and mutant tissue was performed using dCHIP with invariant set normalization.
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Submission date |
Jan 20, 2010 |
Last update date |
Aug 18, 2010 |
Contact name |
Rachel Lockridge Mueller |
E-mail(s) |
rlm@colostate.edu
|
Phone |
(970) 491-6717
|
Organization name |
Colorado State University
|
Department |
Biology
|
Street address |
1878 Campus Delivery
|
City |
Fort Collins |
State/province |
CO |
ZIP/Postal code |
80523-1878 |
Country |
USA |
|
|
Platform ID |
GPL1319 |
Series (1) |
GSE19955 |
Expression data comparing wild-type and spt mutant zebrafish tissues at two developmental time points. |
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