|
| Status |
Public on Nov 14, 2010 |
| Title |
ICN 3-5, DN rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
Day 6 embryoid body
|
| Organism |
Mus musculus |
| Characteristics |
strain: Ainv15
age: Day 6
tissue: sorted cells from embryoid body
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
ICN3-5 DN
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Jan 21, 2010 |
| Last update date |
Jan 21, 2010 |
| Contact name |
Il-Ho Jang |
| E-mail(s) |
ilho_jang@student.hms.harvard.edu
|
| Organization name |
Children's Hospital Boston
|
| Department |
Hematology/Oncology
|
| Lab |
Geoge Daley's lab
|
| Street address |
Karp Bld, rm 7004, 1 Blackfan circle
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE19951 |
Gene expression analysis of murine day 6 embryoid bodies (EBs ) with or without Notch1 (ICN1) induction. |
|
