|
Status |
Public on Mar 23, 2021 |
Title |
Neo3 |
Sample type |
SRA |
|
|
Source name |
Intimal injury 3
|
Organism |
Rattus norvegicus |
Characteristics |
Sex: male strain: Sprague-Dawley (SD) tissue: the carotid artery at 2 wks after intimal injury
|
Treatment protocol |
The intimal injury arteries harvested after 2 wks, and the undamaged right carotid artery served as the self-control, then performed transcriptome sequencing.
|
Growth protocol |
Male Sprague-Dawley (SD) rats with an average weight of 400 g were anesthetized with isoflurane inhalation (MATRX VIP 3000, USA). The left carotid arteries were exposed, and a percutaneous transluminal angioplasty balloon dilatation catheter (2 F, 0.67 mm, Edwards Lifesciences, USA) was used to establish vascular intimal injury (Raugi et al., 1990). The arteries were then harvested after 2 wks, and the undamaged right carotid artery served as the self-control.
|
Extracted molecule |
total RNA |
Extraction protocol |
prefrontal cortex were rharvested , flash frozen on dry ice, and RNA was harvested using Trizol reagent. RNA libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
HiSeq X Ten |
|
|
Description |
N3 Gene expression data from Intimal injury.
|
Data processing |
We applied the reads filtering towards the raw reads after sequencing to achieve the clean data following the criteria: a)10% base quality <15 b) 13% base quality <20. Hisat2 2.0.4 mapping to reference genome count counting Genome_build: Rnor6 Supplementary_files_format_and_content: TXT file contains counts for each sample
|
|
|
Submission date |
Dec 30, 2020 |
Last update date |
Mar 23, 2021 |
Contact name |
Qi yingxin |
E-mail(s) |
baohan123@sjtu.edu.cn
|
Organization name |
shanghai jiao tong university
|
Street address |
NO.800 Dongchuan Road, Shanghai
|
City |
shanghai |
ZIP/Postal code |
200240 |
Country |
China |
|
|
Platform ID |
GPL24688 |
Series (1) |
GSE164050 |
Expression data from the carotid artery at 2 wks after Intimal injury |
|
Relations |
BioSample |
SAMN17186354 |
SRA |
SRX9757874 |