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Sample GSM500965 Query DataSets for GSM500965
Status Public on Jan 23, 2011
Title GSC line 1 (biol rep2)
Sample type RNA
 
Source name Germ Stem Cells OG2
Organism Mus musculus
Characteristics strain: C57BL6
genome/variation: Oct4-GFP transgenic
cell type: germline stem cells (GSCs)
potency: Unipotent
Extracted molecule total RNA
Extraction protocol 400 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 12h).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description cell line 1
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
 
Submission date Jan 25, 2010
Last update date Jan 23, 2011
Contact name Marcos J Ara├║zo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6885
Series (1)
GSE20031 Generation of Autologous Pluripotent Stem Cells from Adult Testis Biopsy

Data table header descriptions
ID_REF
VALUE log2 quantile normalized signal intensity

Data table
ID_REF VALUE
ILMN_1212607 3.772425
ILMN_1212612 3.341367
ILMN_1212619 5.008036
ILMN_1212628 2.126505
ILMN_1212632 3.709472
ILMN_1212636 10.981091
ILMN_1212637 9.781615
ILMN_1212645 2.609832
ILMN_1212648 9.143198
ILMN_1212653 5.126432
ILMN_1212672 7.571262
ILMN_1212682 3.816086
ILMN_1212683 2.848308
ILMN_1212685 2.713213
ILMN_1212692 6.277581
ILMN_1212693 7.790052
ILMN_1212695 2.551244
ILMN_1212698 2.234508
ILMN_1212702 2.675156
ILMN_1212703 5.848264

Total number of rows: 25697

Table truncated, full table size 554 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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