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Sample GSM500968 Query DataSets for GSM500968
Status Public on Jan 23, 2011
Title GSCr line 1
Sample type RNA
 
Source name Germ Stem Cells OG2-ROSA
Organism Mus musculus
Characteristics strain: C57BL6 x 129sv
genome/variation: Oct4-GFP x Lacz transgenic
cell type: germline stem cells (GSCs)
potency: Unipotent
Extracted molecule total RNA
Extraction protocol 400 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 12h).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description cell line 1
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
 
Submission date Jan 25, 2010
Last update date Jan 23, 2011
Contact name Marcos J Ara├║zo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6885
Series (1)
GSE20031 Generation of Autologous Pluripotent Stem Cells from Adult Testis Biopsy

Data table header descriptions
ID_REF
VALUE log2 quantile normalized signal intensity

Data table
ID_REF VALUE
ILMN_1212607 3.990668
ILMN_1212612 2.918371
ILMN_1212619 5.637203
ILMN_1212628 2.305590
ILMN_1212632 4.108379
ILMN_1212636 11.606391
ILMN_1212637 9.701860
ILMN_1212645 3.220495
ILMN_1212648 9.064129
ILMN_1212653 4.933033
ILMN_1212672 7.931436
ILMN_1212682 3.845670
ILMN_1212683 2.835271
ILMN_1212685 2.791166
ILMN_1212692 6.593067
ILMN_1212693 7.759550
ILMN_1212695 2.218769
ILMN_1212698 2.706078
ILMN_1212702 2.566152
ILMN_1212703 5.979273

Total number of rows: 25697

Table truncated, full table size 554 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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