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Sample GSM500969 Query DataSets for GSM500969
Status Public on Jan 23, 2011
Title GSCr line 2
Sample type RNA
 
Source name Germ Stem Cells OG2-ROSA
Organism Mus musculus
Characteristics strain: C57BL6 x 129sv
genome/variation: Oct4-GFP x Lacz transgenic
cell type: germline stem cells (GSCs)
potency: Unipotent
Extracted molecule total RNA
Extraction protocol 400 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 12h).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description cell line 2
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
 
Submission date Jan 25, 2010
Last update date Jan 23, 2011
Contact name Marcos J Ara├║zo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6885
Series (1)
GSE20031 Generation of Autologous Pluripotent Stem Cells from Adult Testis Biopsy

Data table header descriptions
ID_REF
VALUE log2 quantile normalized signal intensity

Data table
ID_REF VALUE
ILMN_1212607 3.330643
ILMN_1212612 2.592791
ILMN_1212619 5.527381
ILMN_1212628 2.528148
ILMN_1212632 6.048983
ILMN_1212636 11.119370
ILMN_1212637 9.727252
ILMN_1212645 3.000262
ILMN_1212648 9.552732
ILMN_1212653 5.433471
ILMN_1212672 8.590162
ILMN_1212682 3.819078
ILMN_1212683 2.604698
ILMN_1212685 2.623445
ILMN_1212692 5.667463
ILMN_1212693 7.513479
ILMN_1212695 2.867411
ILMN_1212698 2.470081
ILMN_1212702 2.398712
ILMN_1212703 5.602245

Total number of rows: 25697

Table truncated, full table size 554 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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