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Sample GSM500970 Query DataSets for GSM500970
Status Public on Jan 23, 2011
Title GSCr line 3
Sample type RNA
Source name Germ Stem Cells OG2-ROSA
Organism Mus musculus
Characteristics strain: C57BL6 x 129sv
genome/variation: Oct4-GFP x Lacz transgenic
cell type: germline stem cells (GSCs)
potency: Unipotent
Extracted molecule total RNA
Extraction protocol 400 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 12h).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description cell line 3
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
Submission date Jan 25, 2010
Last update date Jan 23, 2011
Contact name Marcos J Ara├║zo-Bravo
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
Platform ID GPL6885
Series (1)
GSE20031 Generation of Autologous Pluripotent Stem Cells from Adult Testis Biopsy

Data table header descriptions
VALUE log2 quantile normalized signal intensity

Data table
ILMN_1212607 4.345475
ILMN_1212612 3.382681
ILMN_1212619 5.384400
ILMN_1212628 2.204099
ILMN_1212632 4.531812
ILMN_1212636 11.453765
ILMN_1212637 9.670926
ILMN_1212645 2.485425
ILMN_1212648 9.369000
ILMN_1212653 5.553880
ILMN_1212672 8.393342
ILMN_1212682 4.784698
ILMN_1212683 2.722055
ILMN_1212685 2.239602
ILMN_1212692 6.575019
ILMN_1212693 7.449391
ILMN_1212695 2.603875
ILMN_1212698 2.468169
ILMN_1212702 2.824189
ILMN_1212703 5.749281

Total number of rows: 25697

Table truncated, full table size 554 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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