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Sample GSM500971 Query DataSets for GSM500971
Status Public on Jan 23, 2011
Title ESC rep1 (biol rep2)
Sample type RNA
 
Source name Embryonic Stem Cells
Organism Mus musculus
Characteristics strain: C57BL6
cell type: embryonic stem (ES) cells
potency: Pluripotent
Extracted molecule total RNA
Extraction protocol 400 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 12h).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 1
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
 
Submission date Jan 25, 2010
Last update date Jan 23, 2011
Contact name Marcos J Ara├║zo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6885
Series (1)
GSE20031 Generation of Autologous Pluripotent Stem Cells from Adult Testis Biopsy

Data table header descriptions
ID_REF
VALUE log2 quantile normalized signal intensity

Data table
ID_REF VALUE
ILMN_1212607 3.365107
ILMN_1212612 2.369989
ILMN_1212619 4.409712
ILMN_1212628 2.350676
ILMN_1212632 5.620167
ILMN_1212636 11.463511
ILMN_1212637 9.271107
ILMN_1212645 2.488859
ILMN_1212648 7.966123
ILMN_1212653 3.627909
ILMN_1212672 8.877501
ILMN_1212682 5.521910
ILMN_1212683 2.428991
ILMN_1212685 2.269085
ILMN_1212692 7.687079
ILMN_1212693 3.249864
ILMN_1212695 2.317035
ILMN_1212698 3.889315
ILMN_1212702 3.156227
ILMN_1212703 5.557855

Total number of rows: 25697

Table truncated, full table size 554 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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