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Sample GSM500972 Query DataSets for GSM500972
Status Public on Jan 23, 2011
Title ESC rep2 (biol rep2)
Sample type RNA
Source name Embryonic Stem Cells
Organism Mus musculus
Characteristics strain: C57BL6
cell type: embryonic stem (ES) cells
potency: Pluripotent
Extracted molecule total RNA
Extraction protocol 400 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 12h).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 2
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
Submission date Jan 25, 2010
Last update date Jan 23, 2011
Contact name Marcos J Ara├║zo-Bravo
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
Platform ID GPL6885
Series (1)
GSE20031 Generation of Autologous Pluripotent Stem Cells from Adult Testis Biopsy

Data table header descriptions
VALUE log2 quantile normalized signal intensity

Data table
ILMN_1212607 3.865342
ILMN_1212612 2.194378
ILMN_1212619 5.293220
ILMN_1212628 3.716283
ILMN_1212632 5.890817
ILMN_1212636 11.410254
ILMN_1212637 9.183292
ILMN_1212645 2.519972
ILMN_1212648 7.896575
ILMN_1212653 2.940285
ILMN_1212672 8.922022
ILMN_1212682 2.896454
ILMN_1212683 2.393399
ILMN_1212685 3.407508
ILMN_1212692 8.099888
ILMN_1212693 3.103179
ILMN_1212695 1.826222
ILMN_1212698 2.312730
ILMN_1212702 3.328462
ILMN_1212703 6.089308

Total number of rows: 25697

Table truncated, full table size 554 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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