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Sample GSM500974 Query DataSets for GSM500974
Status Public on Jan 23, 2011
Title gPS line 2 (biol rep2)
Sample type RNA
Source name germ Pluripotent Stem cells from OG2
Organism Mus musculus
Characteristics strain: C57BL6
genome/variation: Oct4-GFP transgenic
cell type: germline-derived pluripotent stem (gPS) cells
potency: Pluripotent
Extracted molecule total RNA
Extraction protocol 400 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 12h).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description cell line 2
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
Submission date Jan 25, 2010
Last update date Jan 23, 2011
Contact name Marcos J Ara├║zo-Bravo
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
Platform ID GPL6885
Series (1)
GSE20031 Generation of Autologous Pluripotent Stem Cells from Adult Testis Biopsy

Data table header descriptions
VALUE log2 quantile normalized signal intensity

Data table
ILMN_1212607 3.772209
ILMN_1212612 3.345309
ILMN_1212619 5.795048
ILMN_1212628 3.727140
ILMN_1212632 4.002157
ILMN_1212636 12.119362
ILMN_1212637 9.086123
ILMN_1212645 2.367143
ILMN_1212648 7.831757
ILMN_1212653 3.432441
ILMN_1212672 8.892909
ILMN_1212682 3.850496
ILMN_1212683 2.414454
ILMN_1212685 3.816320
ILMN_1212692 7.365940
ILMN_1212693 3.016403
ILMN_1212695 2.458697
ILMN_1212698 2.866957
ILMN_1212702 3.297983
ILMN_1212703 5.975400

Total number of rows: 25697

Table truncated, full table size 554 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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