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Sample GSM500976 Query DataSets for GSM500976
Status Public on Jan 23, 2011
Title gPSr line 1
Sample type RNA
 
Source name germ Pluripotent Stem cells from OG2-ROSA
Organism Mus musculus
Characteristics strain: C57BL6 x 129sv
genome/variation: Oct4-GFP x Lacz transgenic
cell type: germline-derived pluripotent stem (gPS) cells
potency: Pluripotent
Extracted molecule total RNA
Extraction protocol 400 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 12h).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description cell line 1
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
 
Submission date Jan 25, 2010
Last update date Jan 23, 2011
Contact name Marcos J Ara├║zo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6885
Series (1)
GSE20031 Generation of Autologous Pluripotent Stem Cells from Adult Testis Biopsy

Data table header descriptions
ID_REF
VALUE log2 quantile normalized signal intensity

Data table
ID_REF VALUE
ILMN_1212607 5.125810
ILMN_1212612 2.702431
ILMN_1212619 4.182944
ILMN_1212628 2.765862
ILMN_1212632 5.052830
ILMN_1212636 11.985332
ILMN_1212637 9.459146
ILMN_1212645 2.115068
ILMN_1212648 7.971978
ILMN_1212653 3.527067
ILMN_1212672 8.972000
ILMN_1212682 4.333153
ILMN_1212683 2.408354
ILMN_1212685 2.124434
ILMN_1212692 5.454151
ILMN_1212693 2.014781
ILMN_1212695 2.143485
ILMN_1212698 2.213163
ILMN_1212702 2.696777
ILMN_1212703 6.551434

Total number of rows: 25697

Table truncated, full table size 554 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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