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Sample GSM500977 Query DataSets for GSM500977
Status Public on Jan 23, 2011
Title gPSr line 2
Sample type RNA
Source name germ Pluripotent Stem cells from OG2-ROSA
Organism Mus musculus
Characteristics strain: C57BL6 x 129sv
genome/variation: Oct4-GFP x Lacz transgenic
cell type: germline-derived pluripotent stem (gPS) cells
potency: Pluripotent
Extracted molecule total RNA
Extraction protocol 400 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 12h).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description cell line 2
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
Submission date Jan 25, 2010
Last update date Jan 23, 2011
Contact name Marcos J Ara├║zo-Bravo
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
Platform ID GPL6885
Series (1)
GSE20031 Generation of Autologous Pluripotent Stem Cells from Adult Testis Biopsy

Data table header descriptions
VALUE log2 quantile normalized signal intensity

Data table
ILMN_1212607 2.598131
ILMN_1212612 2.893488
ILMN_1212619 4.644642
ILMN_1212628 2.682773
ILMN_1212632 6.517571
ILMN_1212636 12.011036
ILMN_1212637 8.507759
ILMN_1212645 2.574617
ILMN_1212648 8.524381
ILMN_1212653 4.582703
ILMN_1212672 9.740777
ILMN_1212682 4.547346
ILMN_1212683 3.023396
ILMN_1212685 2.464242
ILMN_1212692 7.281337
ILMN_1212693 2.409003
ILMN_1212695 2.257738
ILMN_1212698 2.181190
ILMN_1212702 2.584559
ILMN_1212703 5.453117

Total number of rows: 25697

Table truncated, full table size 554 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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