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Sample GSM501329 Query DataSets for GSM501329
Status Public on Jan 23, 2011
Title EtOH_MOLF/EiJ_288
Sample type RNA
 
Channel 1
Source name liver sample from MOLF/EiJ, EtOH
Organism Mus musculus
Characteristics strain: FVB/NJ
tissue: liver
diet: ethanol-containing high fat diet
Treatment protocol A high-fat diet or EtOH-containing high-fat diet was administered via gastric tube for 28 days.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using an RNeasy plus mini kit (Qiagen, Valencia, CA).
Label Cy5
Label protocol 2.0 ug of total RNA from each sample, or 2.5 ug from mouse reference RNA (Icoria, Inc., RTP, NC), was labeled with fluorescent dye (Cy5 and Cy3, respectively).
 
Channel 2
Source name mouse liver RNA (reference)
Organism Mus musculus
Characteristics tissue: liver
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using an RNeasy plus mini kit (Qiagen, Valencia, CA).
Label Cy3
Label protocol 2.0 ug of total RNA from each sample, or 2.5 ug from mouse reference RNA (Icoria, Inc., RTP, NC), was labeled with fluorescent dye (Cy5 and Cy3, respectively).
 
 
Hybridization protocol Labeled samples and reference were hybridized to the microarray at 65 degrees for 17 hours and washed with washing solutions.
Scan protocol Arrays were scanned with Agilent Microarray Scanner (Agilent), and the obtained images were analyzed with Agilent Feature Extraction software (v8.5, Agilent Technologies).
Description Batch5
288
Data processing Normalization and filtering were performed with UNC Microarray Database (https://genome.unc.edu/). Inter-batch normalization was carried out using the ComBat procedure.
 
Submission date Jan 26, 2010
Last update date Jan 23, 2011
Contact name Ivan Rusyn
E-mail(s) iir@unc.edu
Phone 919-843-2596
Fax 919-843-2596
URL http://cancer.unc.edu/research/faculty/displayMember.asp?ID=398
Organization name University of North Carolina
Department Gillings School of Global Public Health
Lab Environmental Genomics Lab
Street address 0031 Michael Hooker Research Center
City Chapel Hill
State/province NC
ZIP/Postal code 27599-7431
Country USA
 
Platform ID GPL7202
Series (1)
GSE20052 Gene expression in the liver of ethanol-treated mice via gastric tube in multiple strains

Data table header descriptions
ID_REF
VALUE Lowess-normalized log2 ratio (sample/reference)

Data table
ID_REF VALUE
A_51_P100063 0.053099589
A_51_P100099 -0.001239367
A_51_P100155 -0.054261868
A_51_P100174 -0.218858188
A_51_P100181 0.275309048
A_51_P100227 -0.005634437
A_51_P100246 0.14480931
A_51_P100289 0.14035668
A_51_P100327 0.035257383
A_51_P100505 0.024696465
A_51_P100565 0.016156065
A_51_P100573 0.025715534
A_51_P100625 0.201477151
A_51_P100787 0.050215185
A_51_P100828 -0.138623152
A_51_P100852 0.086155702
A_51_P100856 0.151939566
A_51_P100866 -0.122513487
A_51_P100991 0.08208502
A_51_P101086 -0.324682402

Total number of rows: 18833

Table truncated, full table size 465 Kbytes.




Supplementary file Size Download File type/resource
GSM501329.txt.gz 14.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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