|
| Status |
Public on Jan 21, 2021 |
| Title |
FA3#-L6 |
| Sample type |
SRA |
| |
|
| Source name |
rabbits liver's DNA
|
| Organism |
Oryctolagus cuniculus |
| Characteristics |
tissue: liver
disease state: rabbit model of hereditary tyrosinemia type I (HT1)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNAs were extracted from the liver tissues of the FAH-/- rabbits injected virus by using TIANamp Genomic DNA Kit (TIANGEN)
Amplification primers containing Illumina forward and reverse adapters were used for a first round of PCR (PCR 1) amplifying the genomic region of interest. Unique Illumina barcoding primer pairs were added to each sample in a secondary PCR reaction (PCR 2).
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
High-throughput sequencing was performed on NOVA SEQ system (Illumina).
Trimmed reads were obtained by removing adapter sequences, and reads with low complexity or of low quality were removed.
The types and proportions of SNVS are analyzed using customized shell scripts
Genome_build: oryCun2
Supplementary_files_format_and_content: comma-delimited csv files include SNV percentage of each Sample …
|
| |
|
| Submission date |
Jan 21, 2021 |
| Last update date |
Jan 25, 2021 |
| Contact name |
Shixue Gou |
| Organization name |
GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH,CHINESE ACADEMY OF SCIENCES
|
| Street address |
No. 190, Kaiyuan Avenue, Huangpu District, Guangzhou
|
| City |
Guangzhou |
| ZIP/Postal code |
510530 |
| Country |
China |
| |
|
| Platform ID |
GPL26786 |
| Series (2) |
| GSE142704 |
CRISPR/Cas9-mediated gene correction in newborn rabbits with hereditary tyrosinemia type I [DeepSeq1] |
| GSE142722 |
CRISPR/Cas9-mediated gene correction in newborn rabbits with hereditary tyrosinemia type I |
|
| Relations |
| BioSample |
SAMN17441181 |
| SRA |
SRX9908687 |
